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En enkel metode til Imaging Arabidopsis Blade Brug Perfluorodecalin som en infiltrativ Imaging Medium
JoVE Journal
Biologia
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JoVE Journal Biologia
A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

En enkel metode til Imaging Arabidopsis Blade Brug Perfluorodecalin som en infiltrativ Imaging Medium

DOI:
10.3791/3394-v

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05:19 min

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January 16, 2012

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,
1Biosciences, College of Life and Environmental Sciences,The University of Exeter

Capítulos

  • 00:05Título
  • 01:54Mounting Leaf Samples in PFD
  • 03:20Representative Leaf Imaging Results
  • 04:37Conclusion

Summary

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Vi beskriver brugen af ​​perfluorodecalin som en infiltrativ montering medium. Dette er en enkel metode til at forbedre dybde af billeddiagnostik<em> Arabidopsis thaliana</em> Blad væv med minimal fysiologisk effekt.

Tags

Arabidopsis LeavesImagingPerfluorodecalinInfiltrative Imaging MediumHigh-resolution ImagesRefractive IndexBiological SamplesSpongy MesophyllPlant LeavesVertebrate LungsFluorescence MicroscopyPerfluorocarbonLaser Scanning Confocal MicroscopyPFD ProtocolOptical ComplexityWater Refractive IndexCytosol Refractive IndexNon-fluorescentNon-toxicSurface Tension

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