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Capítulos
- 00:05Título
- 01:34Obtaining and Staging Metamorphic Flies
- 02:49Steps to Remove Specimen from Puparium
- 03:33Confocal Microscopic Evaluation of Reporter Gene Expression in a Whole Mount Sample
- 04:49Quantifying cis-Regulatory Element Activity with ImageJ
- 05:34Utility of Quantitative CRE Activity
- 07:34Conclusion
Phenotypic variation for traits can result from mutations in cis-regulatory element (CRE) sequences that control gene expression patterns. Methods derived for use in Drosophila melanogaster can quantitatively compare the levels of spatial and temporal patterns of gene expression mediated by modified or naturally occurring CRE variants.
Tags
Cis-regulatory ElementCRE ActivitiesGene Expression PatternsEnhancersCis-regulatory ModulesTranscription Factor ProteinsRegulatory LogicDevelopment ProgramMorphological EvolutionGenetic VariationPhenotypic VariationReporter TransgenesIn Vivo FunctionHeterologous PromoterCoding SequencesReporter GeneProtein ProductP-element Mediated TransgenesisDrosophila Melanogaster
