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随后的三维颅内植入在体内</em生物发光成像鼠脑胶质瘤
JoVE Journal
Medicina
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JoVE Journal Medicina
Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas
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随后的三维颅内植入在体内</em生物发光成像鼠脑胶质瘤

DOI:
10.3791/3403-v

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09:46 min

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November 06, 2011

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, , ,
1Neuro-Oncology Research,Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center, 2Neurosurgery Research Laboratory,Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center

Capítulos

  • 00:05Título
  • 01:32Intracranial Implantation
  • 03:35In Vivo Bioluminescence Imaging
  • 05:243D Imaging
  • 06:18Data Analysis
  • 07:17Representative In Vivo Bioluminescent Imaging Results
  • 09:06Conclusion

Summary

Tadução automática

Tadução automática

颅内植入GL261细胞分化成C57BL / 6小鼠产生恶性胶质瘤，复述人类胶质母细胞瘤的许多特征。我们使用GL261细胞稳定表达荧光素酶，使我们能够使用<em>在体内</em>成像遵循肿瘤进展。手术和3D<em>在体内</em>成像演示。

Tags

Intracranial Implantation3D In Vivo Bioluminescent ImagingMurine GliomasGL261Glioblastoma MultiformeIntracranial Mouse Tumor ModelFirefly LuciferaseGL261-lucGL261-luc2C57BL/6-cBrd/cBrd/Cr MiceAlbino VariantIn Vivo ImagingIVIS SpectrumCaliper Life SciencesHistological FeaturesTumor NecrosisPseudopalisadesNeovascularizationInvasionHypercellularityInflammation

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