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在犁鼻器官的切片准备工作表示一个基因编码的钙传感器成像神经元的反应
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Neurociência
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JoVE Journal Neurociência
Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
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在犁鼻器官的切片准备工作表示一个基因编码的钙传感器成像神经元的反应

DOI:
10.3791/3404-v

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11:26 min

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December 06, 2011

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, , , , ,
1Stowers Institute for Medical Research, 2Department of Anatomy and Cell Biology,The University of Kansas School of Medicine

Capítulos

  • 00:05Título
  • 01:48Preparation of Vomeronasal Organ Slice
  • 04:45Imaging Chamber Setup
  • 06:58Time Lapse Imaging
  • 08:35Data Analysis
  • 09:53Mouse VNO Response to Urine
  • 10:38Conclusion

Summary

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Tadução automática

犁鼻器（VNO）检测种内的化学信号，传达社会和生殖信息。我们已经进行钙离子成像实验，利用转基因小鼠犁鼻组织中表达的G - CaMP2。这种方法可以让我们来分析复杂的犁鼻器神经元的反应模式，以大量的信息素的刺激。

Tags

ImagingNeuronal ResponsesSlice PreparationsVomeronasal OrganGenetically Encoded Calcium SensorChemosensory SignalsSocialSexualReproductive StatusSpeciesPheromonesPredatorsVomeronasal Sensory NeuronsSpecificitySensitivityG-protein Coupled ReceptorsV1RV2RFPRDetection Of Chemosensory CuesPheromone Information EncodingResponse ProfilesStimuliSpecific ReceptorsNeuroepithelia Of VNOVomer BonesSemi-blind Tubular StructureVomeronasal DuctNasal CavityDendritesLumen Part Of VNOPheromone Cues Contact With ReceptorsCell Bodies Of VSNs Form Pseudo-stratified Layers

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