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两种类型的检测，检测青蛙精子Chemoattraction
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Biologia
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JoVE Journal Biologia
Two Types of Assays for Detecting Frog Sperm Chemoattraction
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两种类型的检测，检测青蛙精子Chemoattraction

DOI:
10.3791/3407-v

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10:02 min

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December 27, 2011

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, ,
1Department of Animal Sciences,University of Illinois, Urbana-Champaign, 2School of Life Sciences,Arizona State University

Capítulos

  • 00:05Título
  • 02:42A Two-Chamber Assay for Frog Sperm Chemotaxis
  • 05:46Representative Evaluation of the Chemoattractiveness of Frog Egg Water Proteins
  • 08:22Results: Evaluation of Sperm Trafficking Patterns and Behaviors in Response to a Chemotactic Stimulus
  • 09:40Conclusion

Summary

Tadução automática

Tadução automática

蛋和蛋的周围外涂料频频释放多肽，蛋白质和小分子，与精子的沟通，引导他们到卵子，从而促进受精。利用青蛙的精子，我们描述和比较两类检测，用于检测精子chemoattraction - 精子的积累检测和精子跟踪检测。

Tags

AssaysDetectingFrog SpermChemoattractionInvertebratesVertebratesQuantitative AssaysSperm MovementSperm Accumulation AssaysSwimming TrajectoriesDose Response CurvesTime CoursesNeutrophil ChemotaxisBacterial PeptidesFollicular FluidSperm Tracking AssaysLabor IntensiveSwimming PathsChemoattractant Gradient AxisCharacteristic TurnsChanges In Orientation

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