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监测劈开caspase - 3活性和钾诱导膜去极化使用活细胞成像和Cleaveable荧光染料基板的永生的少突胶质细胞凋亡
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization
DOI:

10:45 min

January 13, 2012

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Capítulos

  • 00:05Título
  • 01:22Culture and Transfection of Oligodendroglial Cells
  • 04:13Preparing Cells for Live-cell Image (LCI)
  • 06:21Treatment of Cells with Apoptosis Inducers and NucView 488 Caspase-3 Substrate
  • 07:20Live-cell Imaging
  • 08:27Cleaved Caspase-3 Actitity after Poassium-induced Membrane Depolarization
  • 10:10Conclusion

Summary

Tadução automática

活细胞成像的caspase - 3介导的细胞凋亡,永生N19少突胶质细胞培养使用<em> NucView</em> 488的caspase - 3基板。这种技术适用于多种类型的细胞和组织细胞程序化死亡实时检测。

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