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Monitoring gekliefde caspase-3 activiteit en apoptose van geïmmortaliseerde Oligodendrogliale cellen met behulp van live-cell imaging en Cleaveable fluorogene-dye Substraten Na Kalium-geïnduceerde membraandepolarisatie
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization
DOI:

10:45 min

January 13, 2012

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Capítulos

  • 00:05Título
  • 01:22Culture and Transfection of Oligodendroglial Cells
  • 04:13Preparing Cells for Live-cell Image (LCI)
  • 06:21Treatment of Cells with Apoptosis Inducers and NucView 488 Caspase-3 Substrate
  • 07:20Live-cell Imaging
  • 08:27Cleaved Caspase-3 Actitity after Poassium-induced Membrane Depolarization
  • 10:10Conclusion

Summary

Tadução automática

Live-cell imaging van caspase-3-gemedieerde apoptose in vereeuwigd N19-oligodendrocyt celculturen met behulp van de<em> NucView</em> 488 caspase-3-substraat. Deze techniek is toepasbaar voor geprogrammeerde celdood testen in real-time in een verscheidenheid van soorten cellen en weefsels.

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