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Monitoraggio spaccati Caspase-3 Attività e apoptosi di cellule oligodendrogliali Immortalata dal vivo utilizzando cellule Imaging e Cleaveable fluorogenici-dye seguenti supporti di potassio indotta depolarizzazione della membrana
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Capítulos
- 00:05Título
- 01:22Culture and Transfection of Oligodendroglial Cells
- 04:13Preparing Cells for Live-cell Image (LCI)
- 06:21Treatment of Cells with Apoptosis Inducers and NucView 488 Caspase-3 Substrate
- 07:20Live-cell Imaging
- 08:27Cleaved Caspase-3 Actitity after Poassium-induced Membrane Depolarization
- 10:10Conclusion
Live-cell imaging di caspasi-3 apoptosi mediata in immortalato N19-oligodendrociti colture cellulari usando il<em> NucView</em> 488 caspasi-3 substrato. Questa tecnica è applicabile per le analisi programmate morte cellulare in tempo reale in una varietà di tipi di cellule e tessuti.
Tags
Cleaved Caspase-3 ActivityApoptosisImmortalized Oligodendroglial CellsLive-cell ImagingCleaveable Fluorogenic-dye SubstratesPotassium-induced Membrane DepolarizationNeurological InsultsNeuronal Population And FunctionDamaged AxonsGlial CellsAstrocytesOligodendrocytesProgrammed Cell DeathSignal Transduction CascadesTraumatic Brain InjuryCerebral IschemiaSeizuresCaspasesChronic Neurodegenerative DiseasesAlzheimer’s DiseaseHuntington’s DiseaseMultiple SclerosisNucView 488 Caspase-3 Substrate
