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RNAi筛选识别胚后的表型 C。线虫</em
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Biologia
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JoVE Journal Biologia
RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
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RNAi筛选识别胚后的表型 C。线虫</em

DOI:
10.3791/3442-v

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09:40 min

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February 13, 2012

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1Department of Molecular and Cellular Medicine,Texas A&M University System Health Science Center

Capítulos

  • 00:05Título
  • 01:24Preparation of Bacteria with Library Clones
  • 03:14Preparation of Nematodes
  • 06:51Observation of Nematodes
  • 08:55Conclusion

Summary

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Tadução automática

我们描述了致敏的方法来确定胚后调节蛋白表达和定位<em> C。线虫</em>使用RNAi技术为基础的基因组的屏幕和一个综合的功能，荧光标记的蛋白质基因的表达。

Tags

RNAi ScreeningC. ElegansPostembryonic PhenotypesModel SystemGenesGene PathwaysFunctional CharacterizationProtocolRNAi ScreenPhenotype AssayFluorescent ProductTransgeneGenomic RNAi LibraryCDNA InsertsRNAi-sensitive BackgroundHatched Animals

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