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Capítulos
- 00:05Título
- 02:02Tissue Preparation
- 03:40Matrix Application
- 05:24MALDI MS Data Acquisition, Processing, and Evaluation
- 12:29Neuropeptide Validation
- 14:18Representative MALDI-MS Results
- 15:55Conclusion
Dopamin udskiftning farmakoterapi anvendelse af L-DOPA er den mest almindeligt anvendte symptomatisk behandling af Parkinsons sygdom, men ledsaget af bivirkninger, herunder ufrivillige unormale bevægelser betegnet dyskinesi<sup> 1</sup>. Her er en protokol til MALDI billedbehandling massespektrometri præsenteret, der registrerer ændringer i rottehjernevæv neuropeptid niveauer relateret til dyskinesi.
Tags
MALDI Imaging Mass SpectrometryNeuropeptidesParkinson’s DiseaseIMSMolecular SpeciesMALDI MatrixIntact PeptidesProteinsMass AnalyzerMALDI TOF/TOF Mass SpectrometerImaging TechniquesMolecules Of InterestUnsupervised AnalysisMolecular SpecificitySensitivityRegion-specific Distribution ProfilesAnimal Model Parkinson’s DiseaseNeurodegenerative DiseaseDopamine ReplacementL-DOPA-induced Dyskinesias (LID)Opioid Precursor Prodynorphin MRNADynorphin PeptidesNeurotransmission
