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Kvantitative levende celler fluorescens-mikroskopi Analyse af Fission Yeast
JoVE Journal
Biologia
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JoVE Journal Biologia
Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Kvantitative levende celler fluorescens-mikroskopi Analyse af Fission Yeast

DOI:
10.3791/3454-v

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06:52 min

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January 23, 2012

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, ,
1Science for Life Laboratory, Department of Medical Biochemistry and Microbiology,University of Uppsala, 2Department of Microbiology,Swedish University of Agricultural Sciences

Capítulos

  • 00:05Título
  • 01:01Fission Yeast Culture and Sample Preparation
  • 03:28Microscopy and Quantitative Measurements
  • 05:03Representative Measurements in Yeast
  • 06:27Conclusion

Summary

Tadução automática

Tadução automática

Den fission gær,<em> Schizosaccharomyces pombe</em>, Er en god model system for at studere de grundlæggende cellulære processer. Her beskriver vi en metode til at udføre kvantitativ levende celler analyse af fission gær. I dette særlige eksperiment har vi fokus på organisering af genomet i cellekernen, men metoden kan også bruges til at studere cytosolisk faktorer.

Tags

Quantitative Live Cell Fluorescence-microscopy AnalysisFission YeastMicroscopy TechniquesSpecific Protein DetectionLive Cell ImagingFluorochromesGreen Fluorescent Protein (GFP)Subcellular LocalizationsProtein MovementsFluorescent MicroscopeSubnuclear LocalizationChromosome StudyLac Repressor Protein (LacR)LacO SequenceGenome VisualizationHomologous RecombinationTargeted IntegrationEngineered Fusion Proteins

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