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浇注和运行的一种蛋白质凝胶重用商业磁带

JoVE Journal
Biologia
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JoVE Journal Biologia

Pouring and Running a Protein Gel by reusing Commercial Cassettes

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浇注和运行的一种蛋白质凝胶重用商业磁带

DOI:

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11:03 min

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February 12, 2012

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Alexander C. Hwang, Paris H. Grey, Katrina Cuddy, David G. Oppenheimer^2,3

¹Department of Biology,University of Florida , ²UF Genetics Institute,University of Florida , ³Plant Molecular & Cellular Biology Program,University of Florida

Capítulos

00:05Título
00:06Introduction
00:48Preparing the Cassettes
01:56Pouring the Resolving Gel
04:01Pouring the Stacking Gel
06:00Storing the Gel
06:32Preparing the Samples
07:35Running the Gel
09:13Staining the Gel
10:17Cleaning the Cassettes
10:35Conclusion

Summary

Tadução automática

我们的协议演示如何倒在时间的多种蛋白质凝胶回收Invitrogen公司Nupage NOVEX微小胶体录音带，并在家装商店购买廉价的材料。这种经济和精简的方法，包括了几个星期的方式储存在4°C凝胶。通过重新使用塑料胶盒从市售凝胶，实验室运行频繁的蛋白凝胶可显着节省成本，并帮助环境。

Tags

Protein Gel Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis SDS-PAGE Analysis Bioquímica Biologia Molecular Polyacrylamide Gels Invitrogen Nupage Novex Minigel Cassettes Cost-saving Method Home Improvement Store Storage Of Protein Gels Environment-friendly Undergraduate Laboratory Classes

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