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醋酸形成醋酸酶激酶活性的直接检测
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Biologia
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JoVE Journal Biologia
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
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醋酸形成醋酸酶激酶活性的直接检测

DOI:
10.3791/3474-v

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05:51 min

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December 19, 2011

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, ,
1Department of Genetics and Biochemistry,Clemson University

Capítulos

  • 00:05Título
  • 01:10Acetyl Phosphate Standard Curve
  • 02:19Assay for Acetate Kinase Activity
  • 03:31The Direct Assay for Acetate Kinase Activity
  • 05:23Conclusion

Summary

Tadução automática

Tadução automática

醋酸激酶活性测定方法的描述。本实验利用一个直接反应在醋酸形成的方向确定不同磷受体酶的活性和乙酸激酶动力学。此外，这种方法可以用于化验等乙酰磷酸盐或乙酰CoA利用酶。

Tags

Acetate KinaseAcetate-forming ActivityEnzymeEnzyme SuperfamilyPhosphorylationATPAcetyl PhosphateBacteriaArchaeaEukaryaMethanosarcina ThermophilaEntamoeba HistolyticaAmoebic DysenteryHydroxamate AssayNADHNAD+Pyruvate KinaseLactate DehydrogenaseInorganic PhosphateHydroxylamineATP FormationADPNADP+NADPHHexokinaseGlucose 6-phosphate Dehydrogenase

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