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血管钙的可视化^{2 +}信号旁分泌源性活性氧引发

JoVE Journal
Biologia

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JoVE Journal Biologia

Visualization of Vascular Ca²⁺ Signaling Triggered by Paracrine Derived ROS

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血管钙的可视化^{2 +}信号旁分泌源性活性氧引发

DOI:

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07:33 min

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December 21, 2011

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Karthik Mallilankaraman, Rajesh Kumar Gandhirajan, Brian J. Hawkins, Muniswamy Madesh

¹Department of Biochemistry,Temple University , ²Department of Anesthesiology and Pain Medicine,University of Washington

Capítulos

00:05Título
01:18Oxidation-sensitive Dyes: Visualization of ROS in J774 Cells
02:24Fluorescent Sensor HyPer: Visualization of ROS in Stably Transfected MPMVECs
04:04Co-Culture Model for Paracrine-Derived ROS
05:13ROS Dynamics
06:50Conclusion

Summary

Tadução automática

一种有效的方法获得可视化的旁分泌衍生活性氧诱导内皮细胞Ca2 +信号的见解的描述。这种方法测量旁分泌衍生活性氧引发共培养模型在血管内皮细胞内Ca2 +动员的优势。

Tags

Vascular Ca2+ Signaling Paracrine ROS (reactive Oxygen Species)Oxidative Stress Ischemia/reperfusion Damage Sepsis Signal Transduction Cell Proliferation Cell Death Inflammatory Cells Cytokines Tumor Necrosis Factor-alpha (TNF-alpha)IL-1 Endothelial Cells (EC)Endothelial Dysfunction Macrophages NADPH Oxidases

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