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Journal / Medicine / Skeletal Muscle Gender Dimorphism from Proteomics
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Skeletal Muscle Gender Dimorphism from Proteomics
 

Skeletal Muscle Gender Dimorphism from Proteomics

Article DOI: 10.3791/3536-v • 09:30 min • December 14th, 2011
December 14th, 2011 •
1, 2, 3, 4
1Center for Proteomics, Smith College, 2Department of Molecular Biophysics and Biochemistry, Yale University, 3Department of Chemistry, Smith College, 4Department of Biological Sciences and Center for Proteomics, Smith College

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A straight-forward set of methods to isolate and determine the identity of the most abundant proteins expressed in skeletal muscle. About 800 spots are discerned on a two-dimensional gel from 10 mg muscle; this allows for the determination of gender-specific protein expression. These methods will give equivalent results in most tissues.

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Skeletal Muscle Gender Dimorphism Proteomics Contractile Proteins Hypertrophy Atrophy Remodeling Adaptations To Stressors Eccentric Contraction Damage To Muscle Repair Degradation Resynthesis Global Proteome Exercise Protein Expression Differential Protein Expression Adaptations To Damage And Repair Male Muscle Female Muscle Two-dimensional Gel Electrophoresis High Resolution Digital Imaging
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