/

/

在开发皮层网络功能钙成像

JoVE Journal
Neurociência
Author Produced

This content is Free Access.

JoVE Journal Neurociência

Functional Calcium Imaging in Developing Cortical Networks

Please note that all translations are automatically generated. Click here for the English version.

在开发皮层网络功能钙成像

DOI:

•

16:33 min

•

October 22, 2011

•

Julia Dawitz, Tim Kroon, J.J. Johannes Hjorth, Rhiannon M. Meredith

¹Department of Integrative Neurophysiology,VU University, Amsterdam

Capítulos

00:03Título
03:00Step 1 Making horizontal hippocampal/cortical slices from young mouse brain
05:57Step 2 Preparation of the staining chamber
08:48Step 3 Staining of slices
10:21Step 4 Non-neuronal dyes and older tissue
12:02Step 5 Attaching slice to recording chamber
13:36Step 6 Imaging
15:58Conclusion

Summary

Tadução automática

神经网络发展的自发活动可以使用AM酯钙敏感的指标染料形式。神经元的激活，细胞内钙的变化，说明被检测为指标荧光瞬态变化与一个或两个光子成像。此协议可适应的发展依赖的神经元网络的范围<em>在体外</em>。

Tags

Functional Calcium Imaging Developing Cortical Networks Synchronized Network Activity Spontaneous Activity Depolarization Voltage-gated Calcium Channels Synchronized Electrical Activity Field Electrodes Patch-clamp Electrophysiology Calcium-dependent Fluorescent Indicator Dyes Spatial Resolution Temporal Sampling

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

同时脑磁图和脑电图的功能映射

在发展中国家斑马鱼的前脑的神经祖细胞克隆相关的定时实时成像

C.在体内神经元钙成像技术线虫

谷氨酸和GABA快速微离子导入：一个有用的工具，调查突触整合

有丝分裂中的不同发育阶段小鼠胚胎皮层实时成像

在基于生物发光钙指标GFP的水母体内脑功能成像方法

自由运动大鼠皮质局部电位、心电图、肌电图和呼吸节律的同步记录

体内新生小鼠皮层神经元的双光子成像

在体内小鼠劣质橄榄中的钙成像

同时脑电图和功能 MRI 期间的可靠脑成像数据获取

Read Article