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Capítulos
- 00:05Título
- 01:07Small Scale Transformation of Bait Plasmid
- 02:12Bait Validation
- 03:09cDNA Library Screening
- 05:06X-gal Assay
- 05:59Retransformation in the MY2H Assay
- 06:47Isolation of Progranulin-Interacting Proteins
- 07:32Conclusion
Tags
Modified Yeast-two-hybrid SystemProteinsInteractingGrowth FactorProgranulinPGRNGranulin Epithelin PrecursorAutocrine Growth FactorPhysiologic ProcessesDisease ProcessesInflammationHost DefenseNeurotrophic FactorPGRN GeneFrontotemporal DementiaCartilage Development And DegradationBinding Receptor(s)Modified Yeast Two-hybrid ApproachGAL4 Based 2-hybrid SystemFalse Positive ClonesReproducible And Reliable System
