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Hele Animal Perfusion Fixation til Gnavere
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Whole Animal Perfusion Fixation for Rodents

Hele Animal Perfusion Fixation til Gnavere

DOI:
10.3791/3564-v

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08:53 min

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July 30, 2012

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, ,
1Biomedical Engineering,University of Michigan , 2Department of Neurological Surgery,University of Washington School of Medicine

Capítulos

  • 00:05Título
  • 01:12Prepare Apparatus
  • 02:58Perfusion Surgery
  • 05:08Perfusion
  • 06:28Brain Dissection and Storage
  • 08:40Conclusion

Summary

Tadução automática

Tadução automática

Her beskriver vi en billig, hurtig, reguleret og ensartet fiksering under anvendelse af 4% paraformaldehyd perfuseres gennem det vaskulære system: gennem hjertet af rotte for at opnå den bedst mulige bevarelse af hjernen.

Tags

Whole Animal Perfusion FixationRodentsFixationImmersion FixationHypoxiaPerfusing FixativeCirculatory SystemVascular NetworkPhysiological PressuresPerfusion Fixation TechniquesParaformaldehyde PerfusionBrain PreservationImmunohistochemistry

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