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测量肽转运到大Unilamellar囊泡
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Biologia
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JoVE Journal Biologia
Measuring Peptide Translocation into Large Unilamellar Vesicles
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测量肽转运到大Unilamellar囊泡

DOI:
10.3791/3571-v

•

12:27 min

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January 27, 2012

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, ,
1Department of Chemistry,Wellesley College

Capítulos

  • 00:05Título
  • 01:37Preparation of Large Unimellar Lipid Vesicles (LUVs)
  • 05:01Quantifying LUV Concentration
  • 08:05Preparing Peptide Solutions
  • 08:46Translocation Quantification
  • 10:37Results: Translocation Assay
  • 11:58Conclusion

Summary

Tadução automática

Tadução automática

该协议的细节肽易位到大量的unilamellar脂质囊泡的定量测量方法。这种方法还提供了关于膜易位率的信息，可用于识别肽，有效地和自发地跨越脂质双层。

Tags

Peptide TranslocationCell-penetrating PeptidesDrug Delivery VectorsAntimicrobial PeptidesMembrane TranslocationPeptide StructureTranslocation AssaysLarge Unilamellar VesiclesLUVsBacterial MembranesEukaryotic Cell MembranesPeptide Fluorescent StudiesMatsuzaki MethodMagaininBuforin IIData Analysis

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