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A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo
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Capítulos
- 00:05Título
- 01:27Donor Cell Transfection
- 03:33Intramyocardial Injection
- 05:34Biophotonic Imaging
- 07:24Biophotonic Imaging Sensitivity Result
- 07:54Conclusion
A method to track cell fusion in living organisms over time is described. The approach utilizes Cre-LoxP recombination to induce luciferase expression upon cell fusion. The luminescent signal generated can be detected in living organisms using biophotonic imaging systems with a sensitivity of detection of ˜1,000 cells in peripheral tissues.
