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Live-תא הדמיה של תאים מעבר הבעת חלבונים fluorescently-מתויג מטריקס תלת מימדי
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Capítulos
- 00:05Título
- 01:00Generation of a Stable Cell Line
- 02:50Silanize the Glass-bottom Dish
- 04:03Preparation of 3D-collagen Gel With Tracer Particles
- 05:35Time-lapse Image Acquisition
- 06:29Inhibitor-addition Experiments
- 07:11FRAP Procedure and Analysis
- 08:15Following Proteins in 3D Cell Migration
- 10:06Conclusion
Tags
Live-cell ImagingMigrating CellsFluorescently-tagged ProteinsThree-dimensional MatrixCell MigrationBiological ProcessesWound HealingCancer MetastasisProtein LocalizationProtein DynamicsExtracellular MatrixOptically Clear MatrixProtein FunctionsGenetically Encoded Fluorescent TagsSubcellular AccumulationCytoskeletal Components
