/
/
Dissectie en Cultuur van Chick Statoacoustic Ganglion en Spinal Cord Explantaten in Collageen Gels voor neurietuitgroei Assays
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 01:10Bead Preparation
- 01:52Statoacoustic Ganglion (SAG) Dissection
- 03:20Spinal Cord Dissection
- 05:28The Explant Culture with Either Purified Proteins or Protein-coated Beads
- 07:53Visualizing of Neurite Outgrowth by Immunohistochemistry
- 09:02Representative Results from Neurite Outgrowth Assays
- 10:42Conclusion
Laten we zien hoe te ontleden en cultuur chick E4 statoacoustic ganglion en E6 ruggenmerg explantaten. Explantaten worden gekweekt onder serum-vrije omstandigheden in 3D collageen gels voor 24 uur. Neurieten responsiviteit is getest met de groei factor-aangevuld medium en met eiwit-gecoate kralen.
Tags
DissectionCultureChick Statoacoustic GanglionSpinal Cord ExplantsCollagen GelsNeurite Outgrowth AssaysSensory OrgansInner EarSAG NeuronsAxon Guidance CuesSurvival FactorsOtic Axons MisroutingGrowth FactorsNeurotrophin-3 (NT-3)Brain-derived Neurotrophic Factor (BDNF)Transgenic AnimalsIn Vitro MethodResponsiveness Of SAG NeuritesSoluble ProteinsMorphogensNeuron SurvivalEmbryonic Day 4 (E4)Three-dimensional Collagen Gels
