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Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
JoVE Journal
Biologia
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JoVE Journal Biologia
Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

DOI:
10.3791/3602-v

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07:15 min

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November 26, 2011

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,
1Department of Chemistry,Tunghai University, 2Department of Biochemistry,University of California, Riverside

Capítulos

  • 00:05Título
  • 02:14Identification of Pepsin-digested Pak2 Fragments
  • 03:34Measurement of Amide H/D Exchange
  • 05:30Time Course of Deuterium Incorporation for Inactive Pak2
  • 06:41Conclusion

Summary

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MALDI-TOF mass spectrometry was successfully utilized to monitor the amide hydrogen/deuterium exchange in protein kinase Pak2 activation.

Tags

Amide Hydrogen/deuterium ExchangeMALDI-TOF Mass SpectrometryProtein-protein InteractionsProtein Conformational ChangesProtein DynamicsProtein-ligand InteractionsDeuteration RatesPepsin DigestionResolutionHeteronuclear Single Quantum Coherence (HSQC)NMRAqueous SolutionMALDI-TOF DetectionHPLC/ESI-MS SystemProtein Kinase Pak2Gamma-Pak

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