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Biologia
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Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
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Biologia
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JoVE Journal Biologia
Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

DOI:
10.3791/3604-v

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07:02 min

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March 11, 2012

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, ,
1Department of Biochemistry and Molecular Biology,University of Arkansas for Medical Sciences

Capítulos

  • 00:05Título
  • 01:34LSD1 Demethylation Assay
  • 03:16Reductive Methylation
  • 04:09MALDI Mass Spectrometry
  • 04:59Data Analysis
  • 05:32MassSQUIRM
  • 06:31Conclusion

Summary

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We present a method for using MALDI mass spectrometry and reductive methylation chemistry to quantify changes in lysine methylation.

Tags

MassSQUIRMQuantitative MeasurementsLysine Demethylase ActivityEpigenetic RegulatorsSmall Molecule StudiesDrug DevelopmentHistonesProteinsDemethylase AssaysQualitative AssaysLysine Methylation StatesMass SpectrometryDifferential IonizationComprehensive AnalysisMassSQUIRM MethodIsotopic Reductive Methylation

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