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Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages
DOI:

10:01 min

March 09, 2012

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Capítulos

  • 00:05Título
  • 02:11Preparation of 1.1% Alginate
  • 02:54Preparation of hESC for Encapsulation
  • 04:03Encapsulation of hESC
  • 05:42Differentiation of Encapsulated hESC to DA Neurons
  • 06:40Decapsulation of Encapsulated hESC
  • 07:28Differentiating DA Neurons in a 3D environment
  • 09:24Conclusion

Summary

Tadução automática

We have optimized a microencapsulation technique as an effective 3D platform for propagation and differentiation of embryonic stem cells to endoderm and dopaminergic (DA) neurons. It also provides an opportunity for immune-isolation of cells from the host during transplantation. This platform can be adapted for other cell types.

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