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A Functional Motor Unit in the Culture Dish: Co-culture of Spinal Cord Explants and Muscle Cells
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Capítulos
- 00:05Título
- 01:07Isolation of the Rat Embryonic Spinal Cord Explants
- 02:39Innervation of the Human Muscle Cells with Embryonic Rat Spinal Cord Explants
- 04:30Co-culture Observations
- 05:27Conclusion
Cultured muscle cells are an inadequate model to recapitulate innervated muscle in vivo. A functional motor unit can be reproduced in vitro by innervation of differentiated human primary muscle cells using rat embryo spinal cord explants. This article describes how co-cultures of spinal cord explants and muscle cells are established.
Tags
Functional Motor UnitCulture DishCo-cultureSpinal Cord ExplantsMuscle CellsAneural Muscle CellsCell DifferentiationMotor Neuron StimulationIn Vitro StudyNeuromuscular DiseasesInnervationPrimary Muscle CellsFiber ContractionMethod DevelopmentDorsal Root GangliaCross-striated Muscle FibersCulture Medium ExchangeHuman Muscle DevelopmentNeuromuscular Junction
