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Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay
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Biologia
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JoVE Journal Biologia
Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

DOI:
10.3791/3619-v

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06:25 min

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February 06, 2012

•

,
1Genes and Environment Laboratory,University of California, Berkeley

Capítulos

  • 00:05Título
  • 01:20Sample Slide Preparation
  • 01:54Localization of Metaphases Using an Automated System
  • 02:25Hybridization
  • 04:26Chromosomics
  • 05:29Conclusion

Summary

Tadução automática

Tadução automática

A novel fluorescence in situ hybridization (FISH) method that simultaneously examines both numerical and structural chromosome alterations, particularly the specific chromosomal translocations associated with leukemia and lymphoma, of all 24 human chromosomes on a single device in one hybridization, is described.

Tags

ChromosomicsNumerical AlterationsStructural AlterationsHuman ChromosomesOctoChrome FISH AssayFluorescence In Situ HybridizationDNA ProbesCytogeneticsChromosome AberrationsBenzene ExposureBenzene-poisoning PatientsHealthy WorkersLeukemia-specific Chromosomal AlterationsCytogentic ChangesBenzene-induced LeukemogenesisSingle FISH AssayWhole ChromosomesSpecific LociMultiple SlidesSpectral KaryotypingWhole Genome Visualization

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