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吸血鬼从细胞外液分离<em>秀丽隐杆线虫</em
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Biologia
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JoVE Journal Biologia
Vampiric Isolation of Extracellular Fluid from Caenorhabditis elegans
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吸血鬼从细胞外液分离<em>秀丽隐杆线虫</em

DOI:
10.3791/3647-v

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08:33 min

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March 19, 2012

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,
1Department of Molecular and Cellular Biology,Harvard University

Capítulos

  • 00:05Título
  • 01:13Preparation of Donor Worms
  • 03:08Preparation of Recipient Worms
  • 03:49Vampiric Isolation of Extracellular Fluid
  • 06:59Representative RNAi Results
  • 08:12Conclusion

Summary

Tadução automática

Tadução automática

模式生物<em> C.线虫</em>使用pseudocoelomic的流体作为被动的循环系统。直接测定该流体一直没有以前可能。在这里，我们提出了一种新的技术直接检测细胞外空间，并使用系统沉默信号在原则的例子作为证明RNAi反应。

Tags

Vampiric IsolationExtracellular FluidCaenorhabditis ElegansModel OrganismGenetic AnalysisMosaic AnalysisCirculatory System CompositionIntercellular SignalingPseudocoelomic FluidMicroinjection TechniquePressure ControlMolecular Assay

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