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تأمين تدفق الأحماض النووية الخلوي ومضان في الموقع التهجين (LNA تدفق FISH) : طريقة لاكتشاف الحمض النووي الريبي البكتيرية الصغيرة
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Capítulos
- 00:05Título
- 01:25LNA Probes and Experimental Design
- 02:31Fixation
- 03:36Diethypyrocarbonate (DEPC) Treatment and Permeabilization
- 04:53Hybridization and Post-hybridization Washes
- 05:47Blocking and Staining
- 07:59Representative Cell Aggregation and LNA flow-FISH Data
- 09:15Conclusion
Tags
Locked Nucleic AcidFlow CytometryFluorescence In Situ HybridizationFISHNucleic Acid SequencesFlow-FISHLysate-based Nucleic Acid Detection MethodsStatistical AnalysisVariance AnalysisTelomere Length DeterminationCellular IdentificationGene ExpressionViral MultiplicationBacterial Community AnalysisDNA Oligonucleotide ProbesNucleic Acid AnalogsSensitivitySpecificityMelting Temperatures (Tm)Locked Nucleic Acid (LNA) Probes
