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Imunologia e Infecção
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Locked Nucleic Acid Flowcytometri-fluorescens In situ Hybridisering (LNA flow-FISH): En metode til bakteriel små RNA Detection
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

Locked Nucleic Acid Flowcytometri-fluorescens In situ Hybridisering (LNA flow-FISH): En metode til bakteriel små RNA Detection

DOI:
10.3791/3655-v

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09:45 min

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January 10, 2012

•

,
1Center for Bio/Molecular Science and Engineering,Naval Research Laboratory

Capítulos

  • 00:05Título
  • 01:25LNA Probes and Experimental Design
  • 02:31Fixation
  • 03:36Diethypyrocarbonate (DEPC) Treatment and Permeabilization
  • 04:53Hybridization and Post-hybridization Washes
  • 05:47Blocking and Staining
  • 07:59Representative Cell Aggregation and LNA flow-FISH Data
  • 09:15Conclusion

Summary

Tadução automática

Tadução automática

En roman high-throughput Metoden er beskrevet som gør det muligt at afsløre og relative kvantificering af små RNA og mRNA udtryk fra enkelt bakterieceller ved hjælp af Locked Nucleic Acid sonder og flowcytometri-fluorescens<em> In situ</em> Hybridisering.

Tags

Locked Nucleic AcidFlow CytometryFluorescence In Situ HybridizationFISHNucleic Acid SequencesFlow-FISHLysate-based Nucleic Acid Detection MethodsStatistical AnalysisVariance AnalysisTelomere Length DeterminationCellular IdentificationGene ExpressionViral MultiplicationBacterial Community AnalysisDNA Oligonucleotide ProbesNucleic Acid AnalogsSensitivitySpecificityMelting Temperatures (Tm)Locked Nucleic Acid (LNA) Probes

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