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GFP的表达和使用的大里基于图像流式细胞仪的可行性评估
JoVE Journal
Biologia
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JoVE Journal Biologia
Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
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GFP的表达和使用的大里基于图像流式细胞仪的可行性评估

DOI:
10.3791/3659-v

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10:58 min

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November 17, 2011

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1Department of Molecular and Cell Biology,Life Technologies , 2Life Technologies

Capítulos

  • 00:05Título
  • 01:26Staining Cells with Tali Viability Kit – Dead Cell Red
  • 02:16Cell Viability Assay Using the Tali Image-Based Cytometer
  • 05:32Setting Thresholds and Gates
  • 09:33Tali Image-Based Cytometry vs. Flow Cytometry
  • 10:12Conclusion

Summary

Tadução automática

Tadução automática

本协议描述了如何使用的大里基于图像流式细胞仪进行细胞活力和荧光表达分析。

Tags

GFP ExpressionViability AssessmentTali Image-based CytometerFlow CytometryFluorescence MicroscopyQuantitative MeasurementsVisualizationImage-based CytometryCell Viability AssayGFP Expression AssayTali InstrumentBenchtop Assay PlatformSuspension Cell-based AssaysApoptosis AnalysisPropidium Iodide

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