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Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

DOI:
10.3791/3673-v

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07:56 min

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September 03, 2012

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, , , ,
1School of Veterinary Medicine and Biomedical Sciences,University of Nebraska-Lincoln, 2Department of Chemistry,University of Nebraska-Lincoln

Capítulos

  • 00:05Título
  • 01:41Culturing M. tuberculosis (Class III Agent)
  • 04:44Adapting the NMR Methodology
  • 06:36Results: M. tuberculosis Wild Type Metabolic Pool
  • 07:16Conclusion

Summary

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The metabolomic profile of Mycobacterium tuberculosis is determined after growth in broth cultures. Conditions can be varied to test the effects of nutritional supplements, oxidants, and anti-tuberculosis agents on the metabolic profile of this microorganism. Procedure for extract preparation is applicable for both 1D 1H and 2D 1H-13C NMR analyses.

Tags

Mycobacterium TuberculosisNuclear Magnetic ResonanceMetabolomic StudiesSample PreparationDrug-resistant StrainsGene FunctionGene Expression AnalysisGlobal PatternsMetabolic CompositionSmall Molecular Weight ChemicalsTarget IdentificationAnti-tubercular ActivityNMR Technology

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