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The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity
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Biologia
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JoVE Journal Biologia
The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity

The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity

DOI:
10.3791/3674-v

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07:00 min

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April 28, 2012

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, ,
1Department of Physiology and Pharmacology, Center for Cardiovascular and Respiratory Sciences,West Virginia University

Capítulos

  • 00:05Título
  • 01:30Preparing Micropipettes and Tie Loops
  • 02:34Harvesting and Cannulating the Microvessel
  • 04:33Microvessel Experiments
  • 05:42Representative Microvessel Results
  • 06:37Conclusion

Summary

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An ex vivo preparation is described for isolation of the largest gracilis muscle resistance arterioles for interrogation of both vascular responses to vasoactive stimuli and the assessment of basic structural properties via passive wall mechanics.

Tags

Ex VivoIsolated Microvessel PreparationVascular ReactivityVessel DiameterPerfusion ResistanceExperimental PreparationGracilis ArterioleRatMechanical ChangesPhysiological ChallengesPharmacological ChallengesIntegrated ResponsesMicrovessel Diameter Regulation

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