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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

DOI:
10.3791/3686-v

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10:41 min

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January 03, 2012

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, , ,
1Department of Molecular Biology and Microbiology,University of Central Florida

Capítulos

  • 00:05Título
  • 01:31Cell Intoxication and Permeabilization
  • 03:34Sample and Standard Toxin Preparation
  • 06:11Surface Plasmon Resonance
  • 07:08Representative Surface Plasmon Resonance Traces and Analysis
  • 10:18Conclusion

Summary

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In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.

Tags

Toxin TranslocationSurface Plasmon ResonanceAB ToxinsCell-binding B SubunitVesicle CarriersEndoplasmic Reticulum (ER)Catalytic A ChainProtein-conducting ChannelCytosolic TargetToxin TraffickingLysosomesGolgi ApparatusSubcellular Fractionation ProtocolDigitonin PermeabilizationAnti-toxin A Chain AntibodyDetection Method

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