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Analytical Techniques for Assaying Nitric Oxide Bioactivity
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JoVE Journal Medicina
Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

DOI:
10.3791/3722-v

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11:28 min

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June 18, 2012

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, , , ,
1Texas Therapeutics Institute,University of Texas Health Science Center at Houston , 2Deptartment of Pediatrics,Baylor College of Medicine

Capítulos

  • 00:05Título
  • 01:59Whole Blood Preparations
  • 03:05Preparing the Plasma for Biochemical Analysis
  • 04:26Preparing the Red Blood Cells for Biochemical Analysis
  • 05:18Tissue Extraction and Preparation
  • 06:02Aortic Ring Isolations
  • 08:10Analyzing Endothelial Function
  • 09:25Dose Response to Acetylcholine
  • 10:11Conclusion

Summary

Tadução automática

Tadução automática

The endogenous production of nitric oxide (NO) regulates a wide variety of biological functions. It is becoming increasingly clear that disruption or dysregulation of NO based signaling is involved in many human diseases. Methods to quantify relevant NO metabolites may provide novel diagnostic or prognostic biomarkers for human disease.

Tags

Analytical TechniquesAssayingNitric Oxide BioactivityDetectionQuantificationSignaling MoleculesBlood Pressure RegulationImmune Response RegulationNeural Communication RegulationAccurate DetectionQuantification In Biological MatricesPhysiological Half LifeAlternative StrategiesDetection Of Reaction ProductsNO MetabolitesBiological CompartmentsIn Vivo NO ProductionBioavailabilityMetabolismWhole Body NO StatusDiagnostic UtilityPrognostic UtilityNO Biomarkers

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