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表型分析和分离小鼠造血干细胞和传承的承诺祖
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Biologia
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JoVE Journal Biologia
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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表型分析和分离小鼠造血干细胞和传承的承诺祖

DOI:
10.3791/3736-v

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12:03 min

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July 08, 2012

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, ,
1Institute for Research in Biomedicine,Bellinzona (Switzerland), 2Dipartimento di Biologia e Genetica per le Scienze Mediche,Universitá degli Studi di Milano

Capítulos

  • 00:05Título
  • 01:45Preparation of Cell Suspension from Bone Marrow
  • 03:49Phenotypic Analysis
  • 06:00Magnetic Enrichment of c-Kit+ Cells
  • 08:11Representative Results
  • 11:24Conclusion

Summary

Tadução automática

Tadução automática

描述的方法来分析流式细胞仪检测骨髓造血祖细胞的分布以及有效地隔离高度纯化造血干细胞（HSCs）。基本上是基于C-kit +细胞和排序，以净化造血干细胞和分子生物学研究细胞的磁富集分离过程。

Tags

Phenotypic AnalysisIsolationMurine Hematopoietic Stem CellsLineage-committed ProgenitorsBone MarrowHSCsBlood Cell LineagesMolecular DissectionHomeostasisHematopoiesisOncologyRegenerative MedicineLabeling ProtocolFluorescent AntibodiesElectronic Gating ProcedureFlow CytometryHematopoietic Progenitor SubsetsDistributionIndividual MiceMagnetic EnrichmentC-Kit ExpressionTrabecular OsteoblastsSinusoidal EndotheliumFunctional NichesN-cadherin+ OsteoblastsReceptor Tyrosine Kinase Tie2Angiopoietin-1

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