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Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
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Medicina
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JoVE Journal Medicina
Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

DOI:
10.3791/3740-v

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10:16 min

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February 10, 2012

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, , , ,
1Department of Pathology, Center for Cardiovascular Biology, and Institute for Stem Cell and Regenerative Medicine,University of Washington, 2Departments of Bioengineering and Medicine/Cardiology,University of Washington

Capítulos

  • 00:05Título
  • 01:27Equipment Preparation
  • 02:18Exposing the Heart and Cannulating the Aorta
  • 04:19Perfusion and Microfil Injection
  • 08:16Representative Microfilled Hearts
  • 09:45Conclusion

Summary

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Tadução automática

Visualization of the coronary vessels is critical to advancing our understanding of cardiovascular diseases. Here we describe a method for perfusing murine coronary vasculature with a radiopaque silicone rubber (Microfil), in preparation for micro-Computed Tomography (μCT) imaging.

Tags

Retrograde PerfusionFillingMouse Coronary VasculatureMicro Computed Tomography ImagingVisualizationImaging TechniquesVascular NetworkResolutionVascular Casting TechniquesMicro-Computed Tomography (µCT)Radiopaque CompoundAccurate RepresentationFilling TechniqueIn Vivo CannulationRetrograde PerfusionLangendorff Perfusion System

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