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Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
 

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

Article DOI: 10.3791/3740-v • 10:16 min • February 10th, 2012
February 10th, 2012 •
1, 1, 1,2, 1, 1
1Department of Pathology, Center for Cardiovascular Biology, and Institute for Stem Cell and Regenerative Medicine, University of Washington, 2Departments of Bioengineering and Medicine/Cardiology, University of Washington

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Visualization of the coronary vessels is critical to advancing our understanding of cardiovascular diseases. Here we describe a method for perfusing murine coronary vasculature with a radiopaque silicone rubber (Microfil), in preparation for micro-Computed Tomography (μCT) imaging.

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Retrograde Perfusion Filling Mouse Coronary Vasculature Micro Computed Tomography Imaging Visualization Imaging Techniques Vascular Network Resolution Vascular Casting Techniques Micro-Computed Tomography (µCT) Radiopaque Compound Accurate Representation Filling Technique In Vivo Cannulation Retrograde Perfusion Langendorff Perfusion System
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