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Profilering tiol Redox Proteome Bruke Isotope Tagging massespektrometri
JoVE Journal
Biologia
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JoVE Journal Biologia
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profilering tiol Redox Proteome Bruke Isotope Tagging massespektrometri

DOI:
10.3791/3766-v

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12:07 min

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March 24, 2012

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, , ,
1Plant Molecular and Cellular Biology Program,University of Florida , 2Department of Biology,University of Florida , 3Interdisciplinary Center for Biotechnology Research,University of Florida , 4Genetics Institute,University of Florida

Capítulos

  • 00:05Título
  • 02:07Inoculation of Tomato
  • 03:13Protein Extraction
  • 04:21Sample Preparation and Peptide Labeling with cysTMT
  • 06:07Removal of Non-reacted Tag Sample Fractionation
  • 07:44Sample Enrichment of cysTMT-labeled Peptides
  • 08:43Mass Spectrometry Analysis
  • 10:02CysTMT Results: Profiling Thiol Redox Proteome of Pseudomonas syringae in Tomato
  • 11:11Conclusion

Summary

Tadução automática

Tadução automática

Reaktive oksygen arter nivå er forhøyet når cellene støter stress forhold. Her viser vi eksempel på 3'-3 'diaminobenzidine farging samt cysTMT merking og massespektrometri for å profilere redoks proteomet i<em> Pseudomonas syringae</em> Behandlet tomatblader.

Tags

Thiol Redox ProteomeIsotope Tagging Mass SpectrometryPseudomonas Syringae Pv. TomatoBacterial Speck DiseaseSolanum LycopersicumBrassica SpeciesArabidopsis ThalianaReactive Oxygen Species (ROS)Hydrogen PeroxideDAB StainingRedox Status Of ProteinsCysteine OxidationTandem Mass Tag (TMT) ReagentsCysteine-reactive TMT (cysTMT) Tags

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