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Capítulos
- 00:01Título
- 00:11Required Materials
- 01:09Step 1. Transferring biopsy from tube to cutting dish
- 02:16Step 2. Dissection of the skin biopsy
- 03:28Step 3. Transfer biopsy pieces to culture plate
- 05:19Representative Results
- 06:38Conclusion
Le protocole de culture d'explants de fibroblastes humains à partir de biopsies de perforation de la peau est une façon techniquement robuste et simple à dériver des cellules de la peau dans les 4-8 semaines pour la banque d'environ 15-20 millions de cellules dans un certain nombre de passage faible.
Tags
Skin Punch BiopsyExplant CulturePrimary Human FibroblastsDisease StudyInduced Pluripotent Stem CellsCell-based Functional AssaysDisease PathwaysDrug ScreeningParkinson’s DiseaseEnzymatic ProceduresReproducibilityTechnical SimplicityTime ConsiderationCell ViabilityAttachment Of CellsDissectionPreparationKeratinocytes
