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简单的微流控芯片的<em在体内</em>成像<em> C.线虫</em><em>果蝇</em>和斑马鱼

JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

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简单的微流控芯片的<em在体内</em>成像<em> C.线虫</em><em>果蝇</em>和斑马鱼

DOI:

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10:32 min

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September 30, 2012

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Sudip Mondal, Shikha Ahlawat, Sandhya P. Koushika²

¹Neurobiology,NCBS-TIFR, ²Department of Biological Sciences,TIFR

Capítulos

00:00Título
00:39Introduction
02:36SU8 Master Fabrication
04:20PDMS Mold Fabrication
06:41Using PDMS Membrane
07:50Inserting C. elegans, Drosophila and Zebrafish Larvae
09:59Conclusions

Summary

Tadução automática

一个简单的微流体装置已经发展到进行麻醉免费<em在体内</em>成像<em> C.线虫</em>完整<em>果蝇</em幼虫和斑马鱼幼虫。该设备采用可变形的PDMS膜固定的模式生物，以执行时间的推移成像的许多过程，如心脏的跳动，细胞分裂和亚细胞神经传输。我们证明了使用本设备，并从不同的模型系统收集的数据的不同类型的显示例子。

Tags

Microfluidic Devices In Vivo Imaging C. Elegans Drosophila Zebrafish Soft Lithography Techniques Sub-cellular Imaging Laser Microsurgery Cellular Imaging Immobilization Techniques Suction Tapered Channels Deformable Membranes Anesthetic-free Immobilization PDMS Device Genetic Model Organisms

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