Journal
/
Bioengenharia
/
在活细胞中的小GTPase活性时空操纵在亚细胞水平上几秒钟的时间刻度
JoVE Journal
Bioengenharia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioengenharia
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Please note that all translations are automatically generated. Click here for the English version.

在活细胞中的小GTPase活性时空操纵在亚细胞水平上几秒钟的时间刻度

DOI:
10.3791/3794-v

•

10:27 min

•

March 09, 2012

•

, , , , , ,
1Department of Cell Biology, Center for Cell Dynamics,Johns Hopkins University, 2Graduate School of Pharmaceutical Sciences,University of Tokyo, 3Biomedical Engineering,Johns Hopkins University

Capítulos

  • 00:05Título
  • 02:17Transfection of Plasmid DNA
  • 04:02Preparation of cRb-A Solution
  • 05:22Microscopy and Light Illumination at the Subcellular Level
  • 07:07Examining the Effect of RAC Activation on Local Ruffle Formation
  • 09:29Conclusion

Summary

Tadução automática

Tadução automática

被描述为轻小GTPase活性的时空控制的方法。这种方法是基于FKBP-FRB雷帕霉素诱导的异二聚体和照片囚禁系统。优化的光照射，使时空，时间控制在亚细胞水平的小GTP酶激活。

Tags

Spatio-temporal ManipulationSmall GTPase ActivitySubcellular LevelSecondsLiving CellsDynamic RegulationRho FamilyGuanosine TriphosphatasesCellular FunctionsVisualizationReal TimePerturbationProtein ActivitiesSubcellular LocationTimingLight-induced ActivationRapamycin-induced FKBP-FRB HeterodimerizationPhoto-caging MethodRapamycinRapid Inducible ActivationInactivationTranslocationPlasma MembraneFRB AnchorPhoto-caging SystemRapamycin Analogs

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

使用介电泳分离珠和多通道微流控芯片细胞和层流

在活细胞中的荧光寿命成像分子转子

氧微环境中的单个胰岛水平的定量和临时控制

从手术组织小学的人结肠癌细胞的分离和培养直接它们柔软的弹性基底牵引术

无标记技术的单细胞分泌物的时空成像

由共轭聚合物活细胞电活动的光控制

活微生物组共培养与人的微工程肠绒毛在肠道上一个芯片微流体装置

人肺泡骨膜间充质干细胞的分离及维生素 D 对骨膜衍生细胞成骨活性的影响

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

荧光技术结合测定活细胞中的力敏感蛋白动力学

Read Article