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实时监控的配体 - 受体相互作用的荧光共振能量转移
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Biologia
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JoVE Journal Biologia
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
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实时监控的配体 - 受体相互作用的荧光共振能量转移

DOI:
10.3791/3805-v

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12:23 min

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August 20, 2012

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, , ,
1Department of Chemistry and Biochemistry,Southern Illinois University

Capítulos

  • 00:05Título
  • 01:50The Concept of FRET
  • 03:16Synthesis of PDA
  • 05:52Liposome Preparation
  • 07:46Preparation of Rhodamine-tagged Bovine Serum Albumin (BSA-Rh) Modified PCDA Liposomes
  • 09:38Representative FRET Results
  • 11:45Conclusion

Summary

Tadução automática

Tadução automática

我们表明FRET之间的共轭聚合物的聚二乙炔（PDA）和荧光基团连接到PDA脂质体的生物分子的传感表面。 PDA脂质体也包含在其表面上的生物分子的受体分子可以用作探针。配体 - 受体相互作用导致这是传感机构的基础的荧光团和PDA之间的FRET效率的变化。

Tags

Real-time MonitoringLigand-receptor InteractionsFluorescence Resonance Energy TransferFRETPDAUV-Vis Electronic Absorption SpectrumAnalyteReceptorsSpectral OverlapJRhodamineFRET EfficiencyDonor FluorophoresModel Protein Molecule StreptavidinCovalent-bindingBovine Serum AlbuminLiposome SurfaceBilayer LiposomesReal-time SensingSmall Chemical MoleculesLarge Biochemical MoleculesDetection Limit

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