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Måling av Cellular chemotaxis med ECIS / Taxis
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Biologia
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JoVE Journal Biologia
Measurement of Cellular Chemotaxis with ECIS/Taxis

Måling av Cellular chemotaxis med ECIS / Taxis

DOI:
10.3791/3840-v

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11:37 min

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April 01, 2012

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, , ,
1Molecular and Cell Biology,University of Connecticut, 2University of Connecticut

Capítulos

  • 00:05Título
  • 01:28ECIS/Taxis Electrode Preparation
  • 03:13Preparing Agarose Chambers and the Cannula Well-cutting Tool
  • 04:21Cutting Wells into Agarose
  • 05:51Loading the Wells
  • 07:04Data Collection
  • 08:55Data Management
  • 10:13Results: Chemotaxis of Human Jurkat T cells in Response to SDF1alpha
  • 11:14Conclusion

Summary

Tadução automática

Tadução automática

Den ECIS / Taxis system er en automatisert, real-time metode som måler mobilnettet chemotaxis. I denne analysen, celler flytte under et lag av agarose å komme fram til et mål elektrode. Cellular bevegelse måles ved utbruddet av motstand mot vekselstrøm 0.

Tags

Cellular ChemotaxisECISTaxisCellularBoyden Chamber AssayUnder-agarose Chemotaxis AssayChemoattractant GradientSolid SubstrateAqueous FilmAgarose LayerAutomated ECIS/Taxis SystemElectric Cell-substrate Impedance SensingTarget ElectrodesCounter-electrode

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