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Capítulos
- 00:05Título
- 01:33Embryo Electroporation
- 03:04Collagen and Slice Culture Medium Preparation
- 04:22Slice Culture Preparation
- 07:52Imaging Embryo Slices
- 08:58Results: Time-lapse Sequences of Spinal Cord Progenitor Cells
- 10:32Conclusion
Billeddannelse embryonisk væv i real-tid er udfordrende over lange tidsperioder. Her præsenterer vi en analyse for at overvåge cellulært og sub-cellulære ændringer i chick rygmarven for lange perioder med høj rumlig og tidsmæssig opløsning. Denne teknik kan tilpasses til andre regioner af nervesystemet og udviklende embryo.
Tags
High-resolutionLive ImagingCell BehaviorDeveloping NeuroepitheliumEmbryonic Spinal CordCycling Neural Progenitor CellsNeuronal DifferentiationMolecular MechanismsNeurogenesisReal-time ImagingCell ViabilityCulturePhototoxic EffectsFluorescent ImagingEx Vivo Slice Culture ProtocolWide-field Fluorescence MicroscopyTime-lapse MonitoringSpatial ResolutionTemporal ResolutionCellular BehaviorsSub-cellular BehaviorsGene Activity ReportersNotch Signaling
