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快速高密度的人类胚胎干细胞培养成纤维细胞去除
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Biologia
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JoVE Journal Biologia
Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
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快速高密度的人类胚胎干细胞培养成纤维细胞去除

DOI:
10.3791/3951-v

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07:12 min

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October 28, 2012

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,
1Department of Engineering,University of California, Merced

Capítulos

  • 00:05Título
  • 01:34Preparation of Mouse Embryonic Fibroblasts (MEF) Feeders
  • 02:21Co-seeding hESCS onto MEFs
  • 03:55Depleting MEFs from the Co-culture
  • 04:50Results: hESCs Purified from Co-culture
  • 06:40Conclusion

Summary

Tadução automática

Tadução automática

尽管转型期文化无饲养层条件下的不断努力，人类胚胎干细胞（胚胎干细胞）的推导和文化仍然在很大程度上依赖于文化与的小鼠胚胎馈线（MEF中）。在这里，我们展示了一种新的方法，快速去除饲养者从人类胚胎干细胞培养实验前。

Tags

Fibroblast RemovalHigh Density Human Embryonic Stem Cell CulturesMouse Embryonic FibroblastsFeeder SystemHESC PurityModified Culture SystemsMitomycin C-treated Mouse Embryonic FibroblastsDifferentiation ExperimentsPurification TechniquesFACSMACSDrug Resistant VectorsPurer Population Of HESCsAspiration Of MEF Sheet

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