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大鼠肠系膜外化：调查涉及在血管生成细胞动力学模型

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis

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大鼠肠系膜外化：调查涉及在血管生成细胞动力学模型

DOI:

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12:29 min

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May 20, 2012

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Ming Yang, Peter C. Stapor, Shayn M. Peirce, Aline M. Betancourt, Walter L. Murfee

¹Department of Biomedical Engineering,Tulane University, ²Department of Biomedical Engineering,University of Virginia , ³Center for Stem Cell Research and Regenerative Medicine,Tulane University

Capítulos

00:05Título
02:06Rat Mesentary Exteriorization Model
04:42Tissue Harvesting and Fixation
06:06Tissue Immunolabeling: Colorimetric Labeling
07:37Tissue Immunolabeling: Fluorescent Labeling
08:27Results: Immunolabeling and Quantitative Analysis of Rat Mesentery Tissues
11:35Conclusion

Summary

Tadução automática

本文介绍了一种刺激的大鼠肠系膜血管生成的简单模型。该模型产生毛细血管发芽，血管面积和血管密度超过在组织允许EN面对整个微血管网络的可视化的单细胞水平的相对较短的时间当然急剧增加。

Tags

Rat Mesentery Exteriorization Cellular Dynamics Angiogenesis Microvascular Network Growth Wound Healing Inflammation Diabetic Retinopathy Tumor Growth Arteriogenesis Spatial Dynamics Temporal Dynamics Vessel Growth Manipulation Reproducible Model Pro-angiogenic Agents Surgical Intervention

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