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Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
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Neurociência
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JoVE Journal Neurociência
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

DOI:
10.3791/3965-v

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10:51 min

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May 24, 2012

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,
1LSU Health Sciences Center – New Orleans, 2Medical School and Stanley S. Scott Cancer Center

Capítulos

  • 00:05Título
  • 01:42Poly-D-Lysine: Preparation and Coating of the Plastic Cell Culture Dishes
  • 03:03PDL and Laminin: Preparation and Coating of the Glass Two-chamber Slides
  • 04:07Neuronal Dissection and Culture
  • 09:40Results: Isolation and Culture of Rat Embryonic Neural Cells
  • 10:27Conclusion

Summary

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We describe a rapid methodology to isolate and culture hippocampal and cortical neurons from rodent embryos. This protocol allows us to perform experiments in which nearly pure neuronal cultures are required.

Tags

IsolationCultureRat Embryonic Neural CellsQuick ProtocolDissociationHippocampal NeuronsCortical NeuronsE15-17 Rat EmbryosMouse Primary NeuronsHuman Primary NeuronsNeural ProgenitorsSerum-free MediumNucleofectionImmunocytochemistryNucleic Acids PreparationElectrophysiologyLentiviral TransductionCalcium Phosphate TransfectionLipid-based Transfection

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