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Oct4GiP Reporter Assay for å studere gener som regulerer Mouse Embryonic Stem Cell Maintenance and Self-fornyelse
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Biologia
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JoVE Journal Biologia
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Oct4GiP Reporter Assay for å studere gener som regulerer Mouse Embryonic Stem Cell Maintenance and Self-fornyelse

DOI:
10.3791/3987-v

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08:01 min

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May 30, 2012

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,
1Laboratory of Molecular Carcinogenesis,National Institute of Environmental Health Sciences

Capítulos

  • 00:05Título
  • 01:38siRNA Transfection in Oct4GiP Cells
  • 04:05FACS Analysis
  • 06:07Results: The Oct4GiP Reporter Assay Readily Detects the Differentiation Caused by Gene Silencing in the Maintenance of ES Cell Renewal
  • 07:30Conclusion

Summary

Tadução automática

Tadução automática

Vi beskriver en fluorescens reporter analyse for å raskt identifisere og karakterisere gener som regulerer mus embryonale stamceller vedlikehold og selvfornyelse.

Tags

Oct4GiPReporter AssayGenesRegulateMouse Embryonic Stem Cell MaintenanceSelf-renewalPluripotencyMolecular MechanismDevelopmental Biology StudiesDisease ModelingDrug DiscoveryRegenerative MedicineFluorescence Reporter-based AssaySelf-renewal StatusMouse ES CellsOct4 Gene Promoter RegionGreen Fluorescent Protein (GFP)DifferentiationFluorescence-activated Cell Sorting (FACS) AnalysisRNAiConvenienceSensitivityQuantitative AnalysisCost-effective

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