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作为一种工具来隔离核糖体的束缚多肽SECM逮捕序列

JoVE Journal
Biologia

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JoVE Journal Biologia

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

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作为一种工具来隔离核糖体的束缚多肽SECM逮捕序列

DOI:

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09:42 min

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June 19, 2012

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Sujata S. Jha, Anton A. Komar

¹Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences,Cleveland State University

Capítulos

00:05Título
03:31DNA Template Preparation, In vitro Transcription and Translation
05:34Isolation of Nascent Polypeptides and Analysis by Tris-tricine SDS PAGE
07:17Representative RNC Results
07:57Conclusion

Summary

Tadução automática

我们在这里描述，现在经常使用的孤立稳定结合的核糖体新生肽链复合物（RNC）中的技术。这种技术发现了17个氨基酸长SECM“逮捕序列”可以停止在原核翻译延伸的优势（<em> E。大肠杆菌</em>）系统，几乎所有的蛋白质（或C-末端融合）插入时。

Tags

SecM Arrest Sequence Ribosome Bound Polypeptides Co-translational Folding Isolation Technique RNCs (ribosome-nascent Chain Complexes)Protein Folding Pathway Conformational Analysis SecM Protein Secretion Monitor SecA ATPase Stalling Sequence Peptidyl-glycyl-tRNA C-terminal Region Polypeptide Chain Elongation In Vivo Studies

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